醫學生社會實踐論文范文10篇

【關鍵詞】Wnt

ExpressionandbiologicalactivityofmouseWnt3ageneinNIH3T3cells

【Abstract】AIM:ToexpressrecombinantmouseWnt3asignalproteinwithbiologicalactivity.METHODS:Therecombinanteukaryoticexpressionplasmid,pSecTag2/HygroBWnt3a,wastransfectedintoNIH3T3cellsbyliposome,thentheexpressedproteinwasdetectedbyWesternBlot.ThesaturationdensityandthecapabilityofsuppressingapoptosisinWnt3a/NIH3T3cellswereevaluated.RESULTS:TheWnt3asignalproteinwasstablyexpressedinWnt3a/NIH3T3cells.ThesaturationdensityandthecapabilityofsuppressingapoptosisinWnt3a/NIH3T3cellsweremarkedlyincreased.CONCLOUSION:WesuccessfullyconstructtherecombinantWnt3asignalproteinwithbiologicalactivity.

【Keywords】Wnt3a;eukaryoticexpression;contactinhibition;apoptosis

【摘要】目的：表達具備生物活性的重組小鼠Wnt3a信號蛋白.方法：應用脂質體轉染試劑將重組真核表達載體pSecTag2/HygroBWnt3a轉染并篩選穩定表達的NIH3T3細胞，WesternBlot鑒定重組Wnt3a蛋白的表達，并對Wnt3a/NIH3T3細胞的融合密度及抗凋亡能力給予檢測.結果：Wnt3a信號蛋白在Wnt3a/NIH3T3細胞中獲得穩定表達，Wnt3a信號蛋白能夠明顯提高NIH3T3細胞的融合密度及抗凋亡能力.結論：在NIH3T3細胞中表達的重組Wnt3a信號蛋白具備生物活性.

【關鍵詞】Wnt3a；真核表達；接觸抑制；細胞凋亡

【關鍵詞】,細胞增殖;,,乳腺癌;,辛基酚；細胞周期蛋白D1;細胞周期蛋白質依賴激酶類

EffectsofoctylphenoloncellcycleandcyclinexpressionofMDAMB231breastcancercells

【Abstract】AIM:Toobservetheeffectsofoctylphenol(OP)oncellcycleandcyclinexpressionofMDAMB231breastcancercellsandtoprobethemolecularmechanismsofOPinhibitingMDAMB231breastcancercellproliferation.METHODS:Thechangesofcellproliferation,cellcycle,cyclinD1mRNA,cyclinD3mRNA,CDK2mRNA,CDK4mRNAandcyclinD1proteinexpressionsofMDAMB231cellstreatedwithOPwereobservedbyusingMTTtest,flowcytometry,immunocytochemistryandRTPCR.RESULTS:WhenMDAMB231breastcancercellsweretreatedwith4or8μmol/LOPfor48h,thecellinhibitionrateswere(17.13±4.1)%,(40.25±8.7)%,andthefluorescencevaluesofcyclinD1were55.1±10.2,41.4±11.2,significantlydecreasedascomparedwithcontrolgroup(89.9±11.2,P<0.05).WhenMDAMB231breastcancercellsweretreatedwith8μmol/LOPfor24and72h,thecellratiosintheG0/G1phasewere(67.0±17.6)%and(44.4±11.9)%，significantlyincreasedascomparedwithcontrolgroup［(46.6±12.8)%at24h,(15.3±3.1)%at72h,P<0.05］.OPinhibitedthemRNAexpressionsofcyclinD1,cyclinD3,CDK2andCDK4andtheproteinexpressionofcyclinD1.CONCLUSION：OPcaninhibittheproliferationofMDAMB231breastcancercells,whichmayberelatedtodownregulatingtheexpressionsofcyclinD1,cyclinD3,CDK2andCDK4mRNAandcyclinD1protein.

【Keywords】cellproliferation;breastneoplasms;octylphenol;cyclinD1；cylindependentkinases

【摘要】目的:觀察辛基酚(OP)對細胞周期及周期蛋白表達的影響，探討OP抑制MDAMB231乳腺癌細胞增殖的可能分子機制.方法：以MTT試驗、流式細胞分析、免疫細胞化學和RTPCR等方法觀察OP對MDAMB231乳腺癌細胞增殖、細胞周期、CDK2，CDK4，cyclinD1和cyclinD3表達的影響.結果：4,8μmol/LOP作用MDAMB231乳腺癌細胞48h時，其抑制率為(17.1±4.1)%,(40.3±8.7)%，cyclinD1表達量熒光值為55.1±10.2，41.4±11.2，比對照組(89.9±11.2)明顯降低(P<0.05).8μmol/LOP作用MDAMB231乳腺癌細胞24h和72h，G0/G1期細胞為(67.0±17.6)%和(44.4±11.9)%，G0/G1期細胞比對照組［24h，(46.6±12.8)%；72h，(15.3±3.1)%］明顯增高(P<0.05).OP導致MDAMB231乳腺癌細胞中CDK2，CDK4，cyclinD1和cyclinD3mRNA的表達降低，且cyclinD1蛋白的表達也降低.結論：OP能抑制MDAMB231乳腺癌細胞增殖，其機制可能與OP能降低乳腺癌細胞中CDK2，CDK4，cyclinD1和cyclinD3mRNA及其蛋白的表達有關.

【關鍵詞】細胞增殖;乳腺癌;辛基酚；細胞周期蛋白D1;細胞周期蛋白質依賴激酶類

【關鍵詞】結直腸腫瘤；低氧誘導因子1α；VHL基因；缺氧誘導因子抑制因子1

EffectofVHLmRNAandFIH1mRNAexpressionsincolorectalcarcinomaonHIF1αproteinlevel

【Abstract】AIM:TodetectmRNAexpressionsofVHLandFIH1incolorectalcancer,andexploretheireffectonHIF1αproteinlevel.METHODS:SemiquantitativeRTPCRassaywasusedtodetecttheexpressionsofVHLmRNA,FIH1mRNAin42patientswithcolorectalcancer;ExpressionofHIF1αproteinwasmeasuredbySPimmunohistochemistry;Spearmanrankcorrelationwasusedtoanalyzethecorrelationbetweenthem.RESULTS:mRNAlevelsofVHLandFIH1incolorectalcancerweresignificantlylowerthanthoseinadjacenttissue（t=-10.14,P=0.00；t=-15.26,P=0.00）；BothexpressionsdecreasedasDukesstageincreased,andtheexpressionsinDukesA+BstageweresignificantlyhigherthanthoseinDukesC+Dstage（t=2.84,P=0.01；t=-5.13,P=0.00）；Theirexpressionshadnorelationshipwithtumorlocationandgender.In42casesofcolorectalcancer,positiverateofHIF1αproteinwas69.1%(29/42),theexpressioninDukesC+Dstage(80.0%,24/30)wassignificantlyhigherthanthatinDukesA+Bstage(41.7%,5/12),(t=5.89,P=0.02);itwasnegativeinadjacenttissue;SpearmanrankcorrelationdisplayedthattheexpressionsofVHLmRNAandFIH1mRNAwerenegativelycorrelatedwiththeHIF1αproteinlevel(rs=-0.97,P=0.01;rs=-0.66,P=0.01).CONCLUSION:TheoverexpressionofHIF1αmayplayanimportantroleinthedevelopmentandmatastasisofcolorectalcancer；ThedecreaseofVHLmRNAandFIH1mRNAexpressionsiscloselycorrelatedwiththeincreaseofHIF1αproteinlevelincolorectalcancer.

【Keywords】colorectalneoplasms;HIF1α;VHL;FIH1

【摘要】目的：檢測大腸癌中VHLmRNA，FIH1mRNA以及HIF1α蛋白的表達,探討VHL，FIH1對HIF1α蛋白水平的影響.方法：采用RTPCR技術檢測42例大腸癌組織和相應的癌旁正常粘膜組織中VHLmRNA，FIH1mRNA的表達，采用免疫組化SP法檢測HIF1α蛋白在大腸癌和癌旁正常組織中的表達，Spearman相關關系檢驗分析其之間的相關性.結果：大腸癌組織VHLmRNA和FIH1mRNA的表達水平顯著低于癌旁正常組織（t=-10.14,P=0.00；t=-15.26,P=0.00）；二者的表達水平隨Dukes分期而降低，DukesA+B期顯著高于DukesC+D期（t=2.84,P=0.01；t=-5.13,P=0.00）；它們的表達水平與與腫瘤部位、性別無關.大腸癌組織HIF1α蛋白表達陽性率為69.1%(29/42),在癌旁正常組織中均為陰性；DukesC+D期(80.0%,24/30)顯著高于DukesA+B期(41.7%,5/12),(t=5.89,P=0.02)；Spearman相關分析顯示，HIF1α表達水平與VHLmRNA，FIH1mRNA的表達水平顯著負相關（rs=-0.97,P=0.01，rs=-0.66,P=0.01）.結論：HIF1α的過表達在大腸癌的發生發展、浸潤轉移過程中起著重要作用，大腸癌組織中VHL以及FIH1的水平下降與HIF1α蛋白水平的升高之間存在密切相關.

【關鍵詞】結直腸腫瘤；低氧誘導因子1α；VHL基因；缺氧誘導因子抑制因子1

【關鍵詞】皮膚腫瘤；免疫組織化學；明膠酶B；抗原CD34；微血管密度

ExpressionofMMP9inskinsquamouscellcarcinomaanditssignificanceinmicroangiogenesis

【Abstract】AIM:TodetecttheroleandthesignificanceofMMP9inmicroangiogenesisinskinsquamouscellcarcinoma(SCC)andtoobservetherelationbetweentheexpressionofMMP9andmicrovesseldensity(MVD)inSCC.METHODS：ImmunohistochemicalSPstainingwasusedtoexamineMMP9expressionandthechangeofMVDunderlightmicroscopein45casesofSCC,19casesofBowendisease,and10casesofnormalskin.RESULTS：TheexpressionofMMP9inSCCgroupwassignificantlyhigherthanthatinBowendiseasegroup（P<0.05）,whilethelatterwasalsosignificantlyhigherthanthatinnormalskingroup（P=0.028，P<0.05）.ThelevelofMVDinSCCgroupwassignificantlyhigherthanthatinBowendiseasegroup（P<0.05）,andthelatterwassignificantlyhigherthanthatinnormalskingroup（P<0.05）.CONCLUSION：MMP9ishighlyexpressedinSCC,andmaybeoneofimportantfactorsinangiogenesisofSCC.

【Keywords】skinneoplasms;carcinoma,squamouscellimmunohistochemistry;gelatinaseB;antigens,CD34;microvesseldensity

【摘要】目的：探討皮膚鱗狀細胞癌（SCC）發生發展過程中基質金屬蛋白酶9（MMP9）對微血管生成的意義.方法：SCC45例,Bowen病19例、正常皮膚10例進行CD34,MMP9免疫組化染色.檢測MMP9的表達和微血管密度（MVD）的變化，分析MMP9的表達與MVD之間，及它們與SCC臨床病理特征之間的關系.結果：在SCC組中MMP9表達高于Bowen病組（P<0.05）,Bowen病組中MMP9表達高于正常皮膚組（P=0.028,P<0.05）.在SCC組中MVD值高于Bowen病組(P<0.05)，Bowen病組中MVD值高于正常皮膚組（P<0.05）.結論：MMP9可能是SCC促進微血管生成因子之一，與SCC的侵襲性相關.

【關鍵詞】皮膚腫瘤；免疫組織化學；明膠酶B；抗原CD34；微血管密度